Abcam, ab72149, Anti-Apc4 antibody

Size: 100µL
Rabbit Polyclonal APC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human ANAPC4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ANAPC4. The exact immunogen used to generate this antibody is proprietary information.Q9UJX5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Apc4 also known as anaphase-promoting complex subunit 4 is a core component of the anaphase-promoting complex/cyclosome (APC/C). This protein has a molecular mass of approximately 92 kDa. It is expressed extensively across various tissues and cells due to its integral role in the regulation of the cell cycle. As part of the APC/C Apc4 plays a mechanical role in mediating ubiquitination and subsequent proteolysis of target cell cycle proteins. This regulation helps ensure proper cell cycle progression particularly the transition from metaphase to anaphase.
Biological function summary
Apc4 is fundamental to maintaining genomic stability by facilitating the APC/C’s role as an E3 ubiquitin ligase. The APC/C is an important multi-subunit complex that governs cell cycle checkpoints by tagging proteins for degradation. Apc4 assists in the recognition and selection of substrates ensuring only specific proteins are degraded. Proper function of the APC/C complex is essential for accurate chromosome segregation and normal mitotic exit.
Pathways
Apc4 functions prominently within the ubiquitin-proteasome pathway and the cell cycle regulation network. Apc4 interacts with key regulatory proteins like Cdc20 and Cdh1 which are necessary for the activation and specificity of the APC/C during different cell cycle phases. Through these interactions it ensures orderly degradation of cyclins and other inhibitors facilitating normal cell cycle progression.
Defects or dysregulation in Apc4-related processes have links to cancers and developmental disorders. In certain cancers aberrant expression or mutation of Apc4 leads to chromosomal instability contributing to tumor progression. Research also suggests its interaction with proteins Cyclin B and Securin which when aberrantly regulated further associates with oncogenic pathways. Understanding Apc4’s involvement in these diseases opens avenues for potential therapeutic interventions.