Abcam, ab72408, Anti-EDC4 antibody

Size: 100µL
Rabbit Polyclonal EDC4 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human EDC4.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:ICC/IF, WB, IHC-P, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human EDC4. The exact immunogen used to generate this antibody is proprietary information.Q6P2E9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EDC4 also known as Enhancer of decapping 4 plays an important role in RNA processing mechanisms. It weighs around 145 kDa. EDC4 is widely expressed in many tissues and cells providing integral support to cellular processes. This protein partners with other decapping proteins in cells to manage RNA turnover ensuring precise degradation of RNA molecules that are no longer needed. Its presence within human tissues suggests its broad involvement in regulating gene expression.
Biological function summary
EDC4 assists in the formation of the decapping complex. This complex facilitates the removal of the 5' cap from mRNAs an essential step in mRNA decay. This decapping activity influences mRNA stability and degradation allowing cells to modulate protein synthesis according to environmental changes. EDC4 interacts with other decapping components like DCP1 further stabilizing its function within the decapping complex and aiding cellular homeostasis.
Pathways
EDC4 plays a part in the mRNA surveillance pathway which ensures the fidelity of gene expression by eliminating faulty mRNA transcripts. Also it participates in the nonsense-mediated decay (NMD) pathway removing mRNA containing premature stop codons. It works alongside proteins such as UPF1 and UPF2 in these pathways to maintain mRNA quality and prevent production of truncated proteins that might disrupt cellular functions.
Alterations in EDC4 expression can relate to neurodegenerative diseases such as Amyotrophic Lateral Sclerosis (ALS) and cancer. In particular the misregulation of mRNA decay processes can contribute to these conditions. EDC4 interacts with proteins like TDP-43 in the context of ALS where disrupted RNA metabolism features prominently in disease pathology. Understanding these interactions offers insight into therapeutically targeting the molecular mechanisms at play in these disorders.