Abcam, ab74136, Anti-PI 3 Kinase p85 alpha + Gamma antibody

Size: 100µg
Rabbit Polyclonal PI 3 Kinase p85 alpha antibody. Suitable for WB, IHC-P, ICC/IF and reacts with African green monkey, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human PIK3R1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, African green monkey,
Applications:IHC-P, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human PIK3R1. The exact immunogen used to generate this antibody is proprietary information.P27986,
Specificity:PI3 kinase p85 alpha and gamma.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PI 3 Kinase p85 alpha + Gamma also known as PI3K is a regulatory subunit of the class IA phosphoinositide 3-kinases. PI3K is comprised of the p85 alpha (approximately 83 kDa) and p110 gamma subunits among others and plays an important role in cell signaling. The p85 alpha subunit is primarily expressed in immune cells liver and muscles while the p110 gamma is mostly found in leukocytes. This kinase is deeply involved in a variety of cellular processes like cell growth proliferation differentiation motility and survival.
Biological function summary
The PI 3 Kinase p85 alpha + Gamma is an important regulator in signal transduction pathways and forms a heterodimer complex with p110 catalytic subunits. This kinase complex is activated by various growth factors and hormones through receptor tyrosine kinases. Upon activation PI3K phosphorylates phosphatidylinositol (45)-bisphosphate (PIP2) to produce phosphatidylinositol (345)-trisphosphate (PIP3) an essential second messenger that recruits downstream signaling proteins like AKT to the plasma membrane. This signal cascade regulates numerous cellular functions affecting homeostasis and adaptive responses.
Pathways
The PI 3 Kinase p85 alpha + Gamma plays a significant role in the PI3K/AKT/mTOR and MAPK signaling pathways. These pathways are essential in controlling cell metabolism survival and growth. Within these cascades PI3K phosphorylates PIP2 to PIP3 creating a docking site for AKT which subsequently becomes activated and relays signals to downstream effectors including mTOR and GSK-3β. The proper functioning of these pathways is necessary for maintaining cellular equilibrium and their dysregulation might lead to pathological conditions.
Aberrant PI 3 Kinase p85 alpha + Gamma activity is implicated in cancer and immune disorders. In particular its malfunction is notable in various cancers including breast and prostate cancer where mutations can lead to constitutive activation of PI3K signaling promoting tumorigenic processes. Additionally mutations or altered expression of PI3K can contribute to immune-related conditions by affecting immune cell function and survival. Interactions between PI3K and other proteins such as PTEN a tumor suppressor represent key nodes in understanding these disease mechanisms and offer potential therapeutic targets.