Abcam, ab74551, Anti-EBNA2 antibody [287C1a]

Size: 100µg
Mouse Monoclonal EBNA2 antibody. Suitable for Dot, WB and reacts with Synthetic peptide - Epstein-Barr virus, Epstein-Barr virus samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:287C1a,
Isotype:IgG1,
Carrier free:No,
Reacts with:Epstein-Barr virus,
Applications:Dot, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum., Storage buffer-pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EBNA2 also known as the Epstein-Barr virus nuclear antigen 2 (EBNA-2) plays a critical role in viral protein function. It is a transcriptional activator essential for the latent infection cycle of Epstein-Barr virus (EBV). EBNA2 binds to specific DNA sequences and interacts with other host cellular factors to initiate transcription. The molecular mass of EBNA2 typically is approximately 88 kDa. EBNA2 mainly expresses in EBV-infected B lymphocytes where it contributes to their immortalization and proliferation.
Biological function summary
EBNA2 is pivotal in the activation of several viral and cellular genes. It forms complexes with other proteins including the cellular transcription factor RBP-Jκ part of the Notch signaling pathway. This interaction is important for altering the expression patterns of both host and viral genes causing changes in the cell cycle and promoting latency. EBNA2 also regulates genes involved in immune response modulation aiding in viral persistence within the host by evading immune surveillance.
Pathways
EBNA2 impacts transcriptional regulation and cell signaling reversibly. It is integral to the Notch signaling and transcriptional activation pathway related to EBV latency. EBNA2 recruits proteins like CBF1 (RBP-Jκ) a transcription factor that helps mediate cell fate decisions and maintain latency. It modulates pathways involving c-MYC a central regulator of cell growth and influences transcriptional activity with a downstream effect on cellular transformation and proliferation.
EBNA2 is prominently associated with EBV-related malignancies such as Burkitt's lymphoma and Hodgkin's lymphoma. These cancers arise partly from the dysregulated cellular growth induced by EBNA2-mediated transcriptional changes. In these disorders EBNA2 may interact with proteins like LMP1 another EBV-encoded protein that aids in the transformation process promoting oncogenesis and affecting therapy resistance mechanisms.