Abcam, ab74555, Recombinant Hantavirus Puumala, strain Vranica, nucleocapsid protein

Size: 100µg
Recombinant Hantavirus Puumala, strain Vranica, nucleocapsid protein is a Full Length protein, expressed in Yeast, with >95%, suitable for ELISA, WB, SDS-PAGE.
Key facts
Purity:>95% SDS-PAGE,
Expression system:Yeast,
Tags:Tag free,
Applications:WB, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Animal free:No,
Carrier free:No,
Reconstitution:Reconstitute at 0.5 mg/mL in PBS,
Storage buffer:Constituents: PBS

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Hantavirus Puumala strain Vranica nucleocapsid also known as N protein performs essential mechanical functions in the virus lifecycle. It plays an important role in encapsidating the viral RNA genome protecting it from degradation and aiding in viral replication. The nucleocapsid protein with a mass of approximately 50 kDa expresses abundantly in infected host cells particularly in rodent reservoir hosts. It forms a ribonucleoprotein complex by binding to the viral genome a critical step in the assembly of new virus particles.
Biological function summary
The nucleocapsid protein impacts viral assembly and host cell interactions. It binds to viral RNA ensuring efficient packaging into new virions essential for successful viral transmission. The nucleocapsid also modulates the host immune response by interfering with the antiviral signaling pathways. Although primarily acting alone the nucleocapsid does engage in interactions with other viral components such as the glycoprotein influencing viral particle morphology and infectivity.
Pathways
The nucleocapsid protein interacts with several molecular processes critical for viral infection and propagation. It significantly impacts the interferon signaling pathway suppressing host innate immune responses to benefit viral replication. The protein also interfaces with cellular pathways involved in RNA synthesis and assembly. Furthermore it shares functional overlap with other viral proteins such as the glycoprotein precursor assisting in the efficient production of infectious virions.
The nucleocapsid protein links closely to hemorrhagic fever with renal syndrome (HFRS) a severe disease affecting humans. The protein's ability to modulate immune responses and facilitate viral replication enhances the pathogenic potential of the virus contributing to the disease's severity. Relations to renal disorders also exist where the protein's interactions with the immune-modulating pathways exacerbate inflammatory responses. Connections with other virus proteins like the glycoprotein are vital in understanding the complete pathology of HFRS and its impact on the host.