Abcam, ab74562, Anti-IDOL antibody

Size: 100µg
Rabbit Polyclonal IDOL antibody. Suitable for ELISA, WB, IHC-P and reacts with Mouse, Human samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human MYLIP.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WB, IHC-P, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MYLIP. The exact immunogen used to generate this antibody is proprietary information.Q8WY64

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IDOL known as Inducible Degrader of the Low-density lipoprotein receptor plays an important role in protein degradation especially the degradation of the LDL receptor (LDLR). The IDOL protein also referred to as MYLIP (Myosin Regulatory Light Chain Interacting Protein) has a molecular mass of approximately 54 kDa. It is widely expressed in tissues with notable expression in the brain liver and macrophages. IDOL functions as an E3 ubiquitin ligase tagging LDLR for degradation which subsequently influences cholesterol levels in the body.
Biological function summary
Within cellular systems IDOL regulates cholesterol homeostasis by controlling the degradation of LDL receptors. It is not directly part of a large protein complex but interacts with other proteins involved in lipid metabolism. By mediating LDLR degradation IDOL indirectly affects cholesterol uptake by cells. IDOL's regulatory mechanism is vital in preventing excessive cellular cholesterol accumulation which can lead to various metabolic problems if unregulated.
Pathways
IDOL serves an important role in the lipid metabolic pathways and is part of the broader cholesterol efflux regulatory network. IDOL acts downstream of the Liver X Receptor (LXR) pathway. The LXR upon activation by oxysterols promotes IDOL expression leading to enhanced LDL receptor degradation. This pathway makes IDOL an important regulator of cholesterol levels linking it with proteins like LDLR and the LXRs. In this regulatory network IDOL ensures balanced cholesterol levels by modulating receptor availability and cholesterol uptake in the liver and peripheral tissues.
IDOL is implicated in conditions such as hypercholesterolemia and atherosclerosis due to its cholesterol regulatory functions. Alterations in IDOL activity or expression can lead to abnormal cholesterol buildup raising the risk of cardiovascular diseases. The connection between IDOL and LDLR is critical as both proteins influence cholesterol metabolism disorders. Mutations or dysregulation affecting IDOL can disturb normal lipid homeostasis consequently contributing to disease progression. Understanding IDOL’s mechanism further opens avenues for potential therapeutic interventions targeting cholesterol-related diseases.