Abcam, ab7468, NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen

Size: 100mL
NBT / BCIP Solution (ab7468 ) is an easy-to-use liquid substrate for alkaline phosphatase (AP) detection in immunoblotting assays e.g. Western blot. - Ready-to-use solution.
Product details:
NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting. Ready to use formulation.
BCIP: 5-bromo-4-chloro-3-indolyl phosphate NBT: p-nitroblue tetrazolium chloride.
This product used to be called "Alkaline phosphatase detection kit (BCIP/NBT)".
NBT / BCIP Solution - Ready to Use Alkaline Phosphatase chromogen is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of NBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.
To use: Wash the membrane after incubation with the alkaline phosphatase conjugate secondary reagent. Apply sufficient substrate to cover the membrane. Color (purple-turquoise, varies with pH) will develop within 30 minutes. Wash, dry membrane and image.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols