Abcam, ab7502, Anti-EBV Latent Membrane Protein 1 antibody [CS1, CS2, CS3, CS4], prediluted

Size: 6mL
Mouse Monoclonal EBV Latent Membrane Protein 1 antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Epstein-Barr virus samples. Cited in 3 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CS1, CS2, CS3, CS4,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Epstein-Barr virus,
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody is specific to 60 kD latent membrane protein (LMP1) encoded by the BNLF1 gene of the EBV. Each clone reacts with different epitopes on the hydrophilic c-terminus of the cytoplasmic domain of LMP1. This antibody stains strongly with EBV positive lymphoblastoid cell lines and EBV infected B cell immunoblasts in infectious mononucleosis.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Purification notes-This product is supplied as a tissue culture supernatant and contains sodium azide as a preservative., Storage buffer-pH: 7.3 - 7.5Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EBV Latent Membrane Protein 1 also known as LMP1 is an important oncoprotein of Epstein-Barr virus (EBV). It acts mechanically to modulate cellular signaling pathways. LMP1 weighs approximately 63 kDa. This protein is highly expressed on the surface of lymphocytes and epithelial cells during latent EBV infection. LMP1 comprises several functional domains including Cytoplasmic Tail 1 (CS1) Cytoplasmic Tail 2 (CS2) and Cytoplasmic Tail 3 (CS3). Researchers often study its presence through methods like LMP1 immunohistochemistry (IHC)
Biological function summary
The protein plays a role in cellular transformation and proliferation acting as a mimic of CD40 receptor signaling. Within cells LMP1 aggregates to form oligomeric complexes that actively recruit TRAF and TRADD adapter proteins. This aggregation leads to the activation of NF-κB signaling and the MAPK pathways contributing to increased cell survival and proliferation. LMP1 functions as a major factor in sustaining EBV latency in infected cells and is essential for the virus's oncogenic potential.
Pathways
LMP1 is an integral component of the NF-κB and AP-1 signaling pathways. By engaging with the host cell machinery LMP1 recruits proteins such as IκB kinase complexes leading to the nuclear translocation of NF-κB transcription factors. This results in the transcription of genes related to cell growth and inflammation. LMP1 also interacts with p38 MAPK further enhancing pathways that contribute to EBV’s ability to foster an environment suitable for its persistence and propagation within the host.
LMP1 has strong associations with various malignancies especially nasopharyngeal carcinoma and Hodgkin's lymphoma. The protein’s role in disrupting normal cellular function relates it closely to the pathology of these cancers. It affects the expression of other proteins like c-Myc and Bcl-2 which are often found altered in malignancy. EBV antibodies particularly those targeting LMP1 are used to study the presence and progression of these diseases in infected individuals.