Abcam, ab75060, Anti-GNPAT/DAPAT antibody

Size: 100µg
Rabbit Polyclonal GNPAT/DAPAT antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 6 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab75060 was purified using an epitope-specific immunogen., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNPAT also known as glyceronephosphate O-acyltransferase or dihydroxyacetone phosphate acyltransferase (DAPAT) is an enzyme that plays an important role in lipid metabolism. The enzyme has a mass of approximately 73 kDa. It is expressed mainly in peroxisomes organelles involved in various metabolic functions. The enzyme catalyzes the initial and rate-limiting step in the biosynthesis of ether lipids like plasmalogens by converting dihydroxyacetone phosphate (DHAP) to acyl-DHAP.
Biological function summary
GNPAT is essential in lipid biosynthesis since ether lipids are integral components of cell membranes and signaling molecules. The enzyme does not work in isolation; it forms part of a multi-enzyme complex that includes other important proteins like alkylglycerone phosphate synthase (AGPS). This association allows GNPAT to efficiently catalyze the first steps in the formation of plasmalogens which serve key roles in protecting cells from oxidative stress and in cell signaling.
Pathways
GNPAT functions efficiently within the ether lipid synthesis pathway which is critical for normal cellular membrane composition and function. The activity of GNPAT impacts the levels of plasmalogens influencing pathways involved in membrane dynamics and antioxidant defense. It works closely with AGPS enhancing the production of essential ether lipids and affects phospholipid metabolism interfacing indirectly with proteins involved in peroxisomal and mitochondrial functions.
GNPAT dysfunction has associations with rhizomelic chondrodysplasia punctata (RCDP) a genetic disorder characterized by skeletal abnormalities and developmental delay. The enzyme's interaction with proteins like Phospholipase A2 is relevant in understanding its role in RCDP. Additionally alterations in plasmalogen levels due to impaired GNPAT function are observed in neurological conditions such as Alzheimer's disease. This connection highlights the importance of GNPAT in maintaining normal brain lipid metabolism and preventing cognitive decline.