Abcam, ab75996, Anti-PRKAR2B antibody [EP2648]

Size: 100µL
Rabbit Monoclonal PRKAR2B antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 5 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP2648,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PRKAR2B also known as cAMP-dependent protein kinase type II-beta regulatory subunit plays a mechanical role by regulating the activity of the protein kinase A (PKA) holoenzyme. With a molecular mass of approximately 46 kDa PRKAR2B serves in anchoring the catalytic subunits in an inactive state in the cytosol. This protein is expressed in various tissues with particularly high levels in the brain. Its presence helps in modulating cellular response to fluctuating levels of cyclic adenosine monophosphate (cAMP).
Biological function summary
PRKAR2B holds a significant role in modulating signal transduction pathways by forming a complex with the catalytic subunits of PKA. This complex becomes active when cAMP binds to the regulatory subunit leading to the release and activation of the catalytic subunits. PRKAR2B's ability to anchor the catalytic subunits places it in central roles in processes like synaptic plasticity and memory formation. It is essential in processes that require precise control of PKA activity allowing response to external stimuli.
Pathways
PRKAR2B integrates into the cAMP signaling pathway and is impactful in the mitogen-activated protein kinase (MAPK) pathway. In the cAMP signaling pathway PRKAR2B regulates the activity of PKA which can phosphorylate numerous target proteins that affect transcription and metabolic regulation. PRKAR2B also interacts with proteins such as AKAP79/150 which target PKA to specific subcellular locations aligning closely with its biological functions within critical pathways.
Alterations in PRKAR2B function or expression have implications in neurological disorders such as schizophrenia and mood disorders. An imbalance in its activity can lead to disrupted cAMP signaling which affects neuronal communication and function. It is related to disorders through its interaction with other proteins such as DARPP-32 which also link to dopaminergic signaling pathways often implicated in these conditions. Research continues to explore PRKAR2B's role in these diseases providing greater understanding of its pathophysiological contributions.