Abcam, ab76470, Anti-CUL-4A + CUL-4B antibody [EPR3200]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Cullin 4B/CUL-4B antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR3200,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Due to the close homology between Cullin 4A and 4B this antibody is likely to react with both forms.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CUL-4A and CUL-4B also known as cullin-4A and cullin-4B are part of the cullin protein family integral to protein degradation processes. These proteins form a scaffold for E3 ubiquitin ligases facilitating the transfer of ubiquitin to substrate proteins marking them for proteasomal degradation. CUL-4A is approximately 87 kDa while CUL-4B is about 104 kDa. Expression is seen in various tissues including testes placenta and brain with CUL-4B having a distinct role in neuronal tissues.
Biological function summary
CUL-4A and CUL-4B contribute significantly to cell cycle regulation and DNA damage response. They are part of the CUL4-DDB1 E3 ubiquitin-protein ligase complexes which handle the ubiquitination of various substrates involved in cell cycle progression and genome stability. These complexes help maintain chromatin structure and secure the genome's integrity by influencing the replication and repair processes ensuring accurate transmission of genetic material.
Pathways
CUL-4A and CUL-4B are significant players in the DNA damage repair and cell cycle control pathways. They collaborate with proteins like DDB1 (damage-specific DNA binding protein 1) and CDT1 (chromatin licensing and DNA replication factor 1) to modulate cell cycle checkpoints and repair mechanisms. Their activity impacts the ATR/ATM pathways ensuring that any DNA damage is effectively managed to prevent genomic instability which could lead to various diseases.
Aberrations in CUL-4A and CUL-4B function have links to cancer and neurological disorders. Changes in their activity can lead to unchecked cell proliferation contributing to tumorigenesis in cancers such as breast cancer. In neurological disorders particularly those affecting brain development abnormal expression of CUL-4B is notably impactful interacting with proteins like DDB1 in both disease progression and development. These associations highlight their importance as potential therapeutic targets.