Abcam, ab76933, Anti-CAST antibody

Size: 100µL
Rabbit Polyclonal CAST antibody. Suitable for ICC, IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human POLR1G aa 400-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human POLR1G aa 400-500. The exact immunogen used to generate this antibody is proprietary information.O15446

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab76933 was affinity purified using an epitope specific to CAST immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Calpastatin also known as CAST is a specific inhibitor of calpain a calcium-dependent cysteine protease. Calpastatin has an approximate mass of 86 kDa and is expressed in various tissues throughout the body including the brain and muscle tissue. Its expression ensures the tight regulation of calpain activity allowing appropriate control of cellular processes which calpain influences through proteolysis.
Biological function summary
Calpastatin acts to regulate calpain's protease activity within cells. It achieves this by directly binding with and inhibiting the activated form of calpain therefore preventing unwanted proteolysis of substrates. Calpastatin does not function as an isolated protein but often forms part of larger protein complexes. Through these interactions calpastatin modulates activities important for cellular homeostasis differentiation and apoptosis.
Pathways
Several cellular pathways integrate calpastatin function owing to its modulation of calpain activity. The calcium signaling pathway significantly involves calpastatin as its inhibition of calpain helps balance cellular responses to changes in calcium ion concentrations. Calpastatin is also closely related to the ubiquitin-proteasome pathway given that calpain can process proteins involved in this regulatory pathway influencing protein degradation and cell cycle progression.
Calpastatin has implications in muscular dystrophies and neurodegenerative diseases. In conditions like muscular dystrophy calpastatin may become dysregulated leading to excessive calpain activity that damages muscle protein structures. Calpastatin's dysregulation is also linked with Alzheimer's disease where aberrant calpain activation appears to contribute to neuronal damage. Understanding how calpastatin interacts with these pathways provides insight into potential therapeutic targets such as calpain in these diseases.