Abcam, ab77398, Anti-Monoacylglycerol Lipase/MGL antibody

Size: 100µg
Goat Polyclonal Monoacylglycerol Lipase/MGL antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human MGLL aa 1-50.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, Flow Cyt (Intra), ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MGLL aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q99685,
Specificity:abb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1). Preliminary testing was unsuccessful on Rat.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab77398 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Monoacylglycerol lipase (MGL) also known as MAGL or lipase 63 is an enzyme with a mass of approximately 33 kDa. MGL primarily functions in lipid metabolism by hydrolyzing 2-monoacylglycerol (2-MAG) into free fatty acids and glycerol. This enzyme is expressed in many tissues with high expression in the brain liver and adipose tissue. Its activity plays a significant role in the breakdown of monoacylglycerol an important intermediate in the endocannabinoid signaling system.
Biological function summary
MGL contributes to the regulation of energy homeostasis by controlling the levels of 2-monoacylglycerol. It catalyzes the final step in the degradation of the endocannabinoid 2-arachidonoylglycerol (2-AG) which is important for modulating inflammation pain and mood. MGL is not known to be part of a larger protein complex. In the brain its action helps terminate endocannabinoid signaling therefore influencing neurotransmission and synaptic plasticity.
Pathways
MGL plays a critical role in the endocannabinoid and glycerolipid metabolism pathways. In the endocannabinoid system it interacts with the cannabinoid receptors CB1 and CB2 by regulating 2-AG levels. The protein's role in glycerolipid metabolism involves its interaction with long-chain acyl-CoA synthetase and fatty acid oxidation pathways which are essential for maintaining lipid balance and cellular energy.
MGL has been linked to neurodegenerative diseases and cancer. In neurodegenerative diseases like Alzheimer’s dysregulation of MGL activity may lead to altered endocannabinoid signaling impacting neuronal health. In cancer increased MGL activity can promote tumor progression by providing cancer cells with fatty acids for growth and survival. MGL's interaction with proteins such as fatty acid synthase highlights its importance in cancer metabolism.