Abcam, ab77586, Anti-ARSA/ASA antibody

Size: 200µL
Goat Polyclonal ARSA/ASA antibody. Suitable for IHC-P, ICC, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARSA aa 400-450.
Key facts
Host species:Goat,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IHC-P, ICC, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARSA aa 400-450. The exact immunogen used to generate this antibody is proprietary information.P15289

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab77586 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide., Storage buffer-pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ARSA protein also known as arylsulfatase A or ASA protein functions mechanically as a lysosomal enzyme responsible for the cleavage of cerebroside sulfate into cerebroside and sulfate. It belongs to a family of enzymes important for the hydrolysis of sulfate esters and has a molecular mass of about 62 kDa. This enzyme is expressed mainly in the liver and kidney but also in the brain where it plays important roles in normal functioning.
Biological function summary
ARSA is essential in the breakdown of sulfatides which are components of myelin sheaths in the central nervous system. It functions as part of a lysosomal complex involving cooperators like saposin B which facilitates its interaction with sulfatide substrates. By breaking down sulfatides ARSA helps in cellular membrane maintenance and recycling contributing to neurological function and stability.
Pathways
ARSA is mainly involved in the sphingolipid metabolism pathway. This pathway manages the degradation of sphingolipids important for maintaining neuron and cell membrane integrity. ARSA also interacts with proteins such as galactosylceramidase as both play roles in lipid recycling and are essential for maintaining cell health within the nervous system.
ARSA's deficiency is a well-known factor in metachromatic leukodystrophy (MLD) a genetic disorder leading to progressive leukodystrophy. This condition results from sulfatide accumulation in the nervous system caused by insufficient ARSA activity. The disease involves other proteins such as saposin B which when dysfunctional exacerbate ARSA-related pathologies and symptoms. Another disorder related to defective ARSA function includes chronic kidney disease where compromised sulfatide metabolism further effects cellular processes.