Abcam, ab7794, Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker

Size: 100µg
Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody detecting 160 kD Neurofilament Medium in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA . Suitable for Cat, Cow, Human, Mouse, Pig, Rat . - KO validated for confirmed specificity - Over 50 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:NF-09,
Isotype:IgG2a,
Light chain type:unknown,
Carrier free:No,
Reacts with:Mouse, Rat, Human, Pig, Cow, Cat,
Applications:ELISA, IHC-P, IHC-Fr, ICC/IF, IHC (PFA fixed), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.

Product details:
What is this antibody validated in?
Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Cat, Cow, Human, Mouse, Pig, Rat samples.
Trusted by the scientific community
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.
Reviewed by scientists
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) has over 5 independent reviews from customers.
Specificity confirmed
The specificity of Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) has been confirmed by Western blot testing in NEFM Knockout HEK-293 cells.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.097% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.
Biological function summary
Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.
Pathways
Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.
Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.