Abcam, ab79172, Anti-SCNN1B (phospho T615) antibody

Size: 100µg
Rabbit Polyclonal SCNN1B phospho T615 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Amiloride-sensitive sodium channel subunit beta phospho T615.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, African green monkey,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Amiloride-sensitive sodium channel subunit beta phospho T615. The exact immunogen used to generate this antibody is proprietary information.P51168

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site., Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCNN1B also known as the beta subunit of the epithelial sodium channel (ENaC) is a critical component in sodium ion transport across epithelial cells. SCNN1B has a molecular mass of approximately 92 kDa. It is primarily expressed in epithelial tissues like the kidney lung and colon where it contributes to maintenance of sodium balance and regulation of fluid homeostasis. The protein plays an essential role in controlling sodium absorption which is necessary for various physiological processes.
Biological function summary
SCNN1B is a part of the epithelial sodium channel complex which includes two other subunits: SCNN1A (alpha) and SCNN1G (gamma). Together with these subunits SCNN1B facilitates the selective transport of sodium ions impacting processes such as blood pressure regulation and airway surface liquid homeostasis. The channel formed by these subunits operates under guidance by aldosterone a hormone that influences its assembly and activity.
Pathways
SCNN1B takes part in the Renin-Angiotensin-Aldosterone System (RAAS) and sodium reabsorption pathways. These are vital for maintaining electrolyte balance and blood pressure regulation. SCNN1B interacts closely with proteins such as Nedd4-2 which modulates its activity by influencing channel degradation. This interaction ensures that sodium transport and fluid balance remain tightly regulated.
Abnormalities in SCNN1B function relate to conditions like Liddle syndrome and hypertension. Mutations causing hyperactivity of the channel can lead to excessive sodium reabsorption resulting in these conditions. Furthermore SCNN1B has connections with other proteins like SGK1 which plays a regulatory role in adapting sodium channel activity. Understanding these links is important for targeting these conditions with medical therapies.