Abcam, ab79654, Biotin Anti-HMW Kininogen antibody

Size: 150µg
Rabbit Polyclonal Kininogen antibody - conjugated to Biotin. Suitable for IP, ELISA, WB, RIA and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human KNG1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Conjugation:Biotin,
Carrier free:No,
Reacts with:Human,
Applications:RIA, WB, IP, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human KNG1.P01042

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.5Preservative: 0.01% Thimerosal (merthiolate)Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
High molecular weight kininogen also known as HMW kininogen or HK is a glycoprotein with a molecular mass of about 120 kDa. It is synthesized in the liver and circulates in the bloodstream. It serves as a precursor to bradykinin a peptide involved in inflammation blood pressure regulation and pain. HMW kininogen also acts as a cofactor for kallikrein activation playing a significant role in the intrinsic pathway of blood coagulation. Additionally it exists as part of a quaternary structure with other plasma proteins functioning within the circulatory system.
Biological function summary
This kininogen is essential for the kallikrein-kinin system and interacts with various cell surface receptors to trigger diverse cellular responses. It is part of a complex that includes prekallikrein and factor XII facilitating the assembly and activation of these components on negatively charged surfaces. This interaction supports the generation of bradykinin which mediates vasodilation and increases vascular permeability critical processes in inflammatory responses. The multifunctional nature of HMW kininogen allows it to contribute broadly to the regulation of hemostasis and innate immunity.
Pathways
HMW kininogen is linked notably to both the contact activation pathway and the renin-angiotensin system. In the contact activation pathway it associates closely with prekallikrein and factor XII promoting the conversion of prekallikrein to kallikrein and subsequent steps in coagulation. In the renin-angiotensin system bradykinin produced from HMW kininogen modulates blood pressure through interactions with ACE (angiotensin-converting enzyme) showcasing the enzyme's relationship with these pathways. These pathways position HMW kininogen at a critical junction of cardiovascular and immune system regulation.
HMW kininogen associates with conditions such as hereditary angioedema and cardiovascular disorders. Hereditary angioedema results from bradykinin overproduction implicating HMW kininogen in its pathophysiology with links to defects in C1 inhibitor another regulatory protein. Cardiovascular disorders may involve aberrant bradykinin production and metabolism where HMW kininogen plays a role through interaction with the renin-angiotensin system. Aberrations in its function or expression can contribute toward the pathogenesis of these conditions highlighting the need for further research into its clinical implications.