Abcam, ab81484, Recombinant mouse Wnt3a protein

Size: 10µg
Recombinant mouse Wnt3a protein is a Mouse Full Length protein, in the 19 to 352 aa range with >75% purity, < 1 EU/µg endotoxin level and suitable for cell differentiation assays, HPLC, and SDS-PAGE. The predicted molecular weight of ab81484 protein is 37.4 kDa. - Save time and ensure accurate results- use our recombiant Wnt3a protein as a control - Optimal protein bioactivity, stability and reproducibility - Cited in over 15 publications
Key facts
Purity:>75% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Cell Culture,
Tags:Tag free,
Applications:SDS-PAGE, CellDiff, CellAct, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The ED50 was determined by its ability to induced alkaline phosphatase production by CCL-226 cells.The expected ED50 for this effect is 0.4-0.6 ng/ml.,
Accession:P27467,
Animal free:No,
Carrier free:No,
Species:Mouse,
Reconstitution:reconstitute with water at 0.1mg/mL,
Storage buffer:Constituents: 13% 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

Product details:
Ensure the validity of your results using our Recombinant mouse Wnt3a protein ab81484 as a positive control in SDS-PAGE.
Our bioactive recombinant mouse Wnt3 protein ab81484 is ideal to use in oncogenesis research and several developmental processes, including regulation of cell fate and patterning during embryogenesis.
The ab81484 mouse Wnt-3a is a monomeric glycoprotein. Due to glycosylation ab81484 migrates at an observed molecular weight of ~38-41 kDa by SDS-PAGE under non-reducing conditions.
Check out our protein gel staining guide for SDS-PAGE

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-A few weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.
Biological function summary
Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.
Pathways
Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.
Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.