Abcam, ab818, Anti-TATA binding protein TBP antibody [1TBP18] - Loading Control and ChIP Grade

Size: 100µg
Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) is a mouse monoclonal antibody detecting TATA binding protein TBP in Western Blot, Flow Cytometry, IP, IHC-P, IHC-Fr, ICC/IF, ELISA, EM, ChIP . Suitable for Human, Mouse, Rat . - Over 260 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1TBP18,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, Mouse, Rat,
Applications:Flow Cyt, ChIP, IHC-P, ELISA, ICC/IF, IHC-Fr, WB, IP, ChIP/Chip, ICC, EMSASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TBP. The exact immunogen used to generate this antibody is proprietary information.P20226,
Epitope:Within amino acid residues 1-20 of human, mouse and rat TBP.The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.

Product details:
What is this antibody validated in?
Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Electron microscopy (EM), ChIP in Human, Mouse, Rat samples.
What is the molecular weight of TATA binding protein TBP?
Anti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) specifically detects a band for TATA binding protein TBP (UniProt: P20226) at a molecular weight of 38kDa.
Trusted by the scientific community
Anti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) was first used in a scientific publication in 2002 and has been cited over 260 times in peer-reviewed journals.
Reviewed by scientists
Anti-TATA binding protein TBP [1TBP18] - ChIP Grade (ab818) has over 35 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TATA binding protein (TBP) also known as TBP or TFIID (Transcription Factor IID) is a central player in transcription initiation. This protein weighing approximately 38 kDa binds to the TATA box a DNA sequence found in the promoter region of many genes especially those transcribed by RNA polymerase II. TBP facilitates the loading of the transcription machinery onto DNA enabling the start of transcription. It is found inside the nucleus of eukaryotic cells present across diverse cell types owing to its role in gene expression.
Biological function summary
TBP serves as a pivotal anchor in the assembly of the transcription preinitiation complex contributing to the process by bending DNA to assist other transcription factors such as TFIIA and TFIIB in binding. Its role in this complex illustrates its importance in regulating gene expression at a fundamental level. TBP’s interaction with other transcription factors ensures precise transcription regulation reflecting its significant contribution to cellular activities.
Pathways
TBP acts within the RNA polymerase II transcription initiation pathway and is integral in regulating gene expression. TBP's involvement is critical for the process of starting the transcription of mRNA. In its pathway TBP interacts with proteins like TFIID helping to anchor other transcription factors in the complex. By collaborating with these proteins TBP shapes gene regulatory networks essential for a wide range of cellular functions.
TBP is linked to conditions such as spinocerebellar ataxia type 17 (SCA17) and Huntington's disease. SCA17 is associated with mutations in the TBP gene that disrupt its usual functioning leading to neurodegeneration. In Huntington's disease TBP's interaction with proteins such as huntingtin may influence nuclear signaling pathways involved in disease progression. The understanding of TBP’s role in these disorders underlines its relevance in research focused on genetic diseases and transcription-related pathologies.