Abcam, ab83205, Recombinant Human Rb protein

Size: 5µg
Recombinant Human Rb protein is a Human Full Length protein, in the 1 to 928 aa range, expressed in Baculovirus, with >95% purity and suitable for western blot, SDS-PAGE, GSA and EMSA.The predicted molecular weight of ab83205 protein is 107.5 kDa. - Save time and ensure accurate results- use our Retinoblastoma protein (Rb) as a control
Key facts
Purity:>95% SDS-PAGE,
Expression system:Baculovirus,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, WB, GSA, EMSASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P06400,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.9Constituents: 20% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA

Product details:
Ensure the validity of your result using our recombinant human Retinoblastoma protein (Rb/Rb1/pRb) protein ab83205 as a positive control in western blot and SDS-PAGE.
The Rb protein ab83205: 1 nanogram of purified protein is sufficient for a gel mobility shift assay (GSA) in a 20 µl reaction; 50 nanogram are sufficient for reconstituted transcription assay and 100 nanogram units are sufficient for a protein-protein interaction assay
Check out our protein gel staining guide for SDS-PAGE
Check out of western blot protocol for more information

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Retinoblastoma protein (Rb) also known as pRb is an important regulatory protein with a molecular weight of approximately 110 kDa. It is mainly expressed in the nucleus of cells. Rb functions as a tumor suppressor by controlling the cell cycle progression from G1 to S phase. Rb becomes active when it is dephosphorylated allowing it to bind and inhibit E2F transcription factors consequently preventing the transcription of genes essential for S phase entry.
Biological function summary
Retinoblastoma protein influences cellular proliferation differentiation and apoptosis. It acts within a larger protein complex modulating various cellular responses. When functional Rb halts uncontrolled cell division important for maintaining normal tissue homeostasis. In its phosphorylated state known as phospho-Rb or phospho-Rb E182 it loses its regulatory capabilities which can lead to unrestrained cell cycle progression.
Pathways
Several involve the retinoblastoma protein. One key pathway is the p53 pathway which Rb interacts with to influence cellular outcomes like cell-cycle arrest and apoptosis. Rb cooperates with proteins like p21 to implement these processes. Additionally it is involved in the cyclin D-dependent kinase 4 (CDK4) pathway which modulates its phosphorylation state influencing binding interactions and cell cycle control.
Dysregulation of retinoblastoma protein is commonly associated with cancer particularly retinoblastoma and breast cancer. In retinoblastoma mutations in the Rb gene directly lead to uncontrolled cell proliferation due to the absence of functional Rb protein. Additionally Rb's connection to the E2F family of transcription factors can become disrupted contributing to oncogenesis in other cancers.