Abcam, ab84072, Recombinant human Klotho protein (Active)

Size: 5µg / 20µg
Recombinant human Klotho protein (Active) is a Human Fragment protein, in the 34 to 549 aa range, expressed in CHO, with >98% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab84072 protein is 58.6 kDa. - Save time and ensure accurate results - use our Klotho protein as a control - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs
Key facts
Purity:>98% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:CHO cells,
Tags:Tag free,
Applications:HPLC, FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Determined by the dose-dependent stimulation of the proliferation of murine NIH/3T3 cells.ab84072 is effective in a concentration range of 0.5-2.0 μg/ml.,
Accession:Q9UEF7,
Animal free:No,
Carrier free:No,
Species:Human,
Reconstitution:Reconstitute in water

Product details:
Ensure the validity of your result using our bioactive recombinant human Klotho (KL) protein ab84072 as a positive control in SDS-PAGE.
The rKlotho ab84072 predicted MW is 58.6 kDa and migrates at an apparent molecular weight of 65- 70 kDa by SDS-PAGE analysis under reducing conditions.
ab84072 endotoxin level is less than 0.1 ng per μg (1EU/µg)
Check out our protein gel staining guide for SDS-PAGE
Check out of western blot protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.
Biological function summary
Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.
Pathways
The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.
Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.