Abcam, ab84864, Anti-AZI1 antibody

Size: 100µL
Rabbit Polyclonal AZI1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CEP131 aa 1000 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human CEP131 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9UPN4

Product details:
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Purified using an epitope specific to AZI1 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AZI1 also known as 5-azacytidine-induced protein 1 is a protein involved in various cellular processes. It has a molecular weight of approximately 52 kDa. AZI1 is expressed in multiple tissues with higher expression levels reported in the testis and basal skin epidermis. This protein is known for its mechanistic role in the regulation of ciliogenesis contributing to the formation and function of cilia in the cell. While the precise mechanisms remain under study AZI1's presence in cilia is significant for its mechanical functions.
Biological function summary
The function of AZI1 is associated with its participation in the BBSome complex a group of proteins critical for proper ciliogenesis. It acts as an important player in ciliogenesis aiding in the assembly and maintenance of cilia which are hair-like structures on cells. Cilia play essential roles in cellular signaling and the transport of proteins and other molecules across cellular surfaces. AZI1 helps in the recruitment of proteins essential for the biogenesis and maintenance of these structures thereby ensuring the correct functioning of cilia-dependent processes.
Pathways
AZI1's involvement in ciliogenesis makes it integral to the Hedgehog signaling pathway which relies heavily on ciliary function for the transduction of signals that regulate cellular growth and differentiation. In addition AZI1 interacts with proteins such as DYF-2 a homolog of human IFT144 which also participates in ciliogenesis and is a component of intraflagellar transport linking AZI1 to the intraflagellar transport pathways necessary for maintaining cilia structure and function.
AZI1's association with ciliogenesis connects it to disorders like Bardet-Biedl syndrome (BBS) a condition characterized by defects in ciliary structure and function. The malfunctioning of AZI1 and related BBSome proteins disrupts normal cilia function contributing to the development of BBS which manifests in symptoms like vision loss and obesity. Furthermore mutations in AZI1 are also studied in the context of certain ciliopathies connecting it alongside proteins like BBS1 and BBS4 which are also involved in these syndromes. Understanding AZI1's role in these disorders enhances insights into therapeutic targets for cilia-related diseases.