Abcam, ab85486, Recombinant Human Chromogranin A protein

Size: 50µg / 250µg
Recombinant Human Chromogranin A protein is a Human Full Length CHGA protein, in the 19 to 457 aa range with >80% purity and suitable for western blot and SDS-PAGE. The observed molecular weight of ab85486 recombinant protein will appear higher than 51.2 kDa. - Save time and ensure accurate results - use our recombinant Chromogranin A (CHGA) protein as a control - Available in different sizes to fit your experimental needs
Key facts
Purity:>80% SDS-PAGE,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:WB, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P10645,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.5Constituents: 10% Glycerol (glycerin, glycerine), 0.39% MES, 0.0584% EDTA, 0.00174% PMSF

Product details:
Ensure the validity of your result using our recombinant human Chromogranin A protein ab85486 as a control:
Chromogranin A protein ab85486 can be used as a positive control in SDS-PAGE and western blot assays.
Analyze the Chromogranin A protein levels in your ELISA data using the ab85486 to generate and plot the standard curve.
In Western blot ab85486 antibody can be used to quantify Chromogranin A protein expression in tissues under different experimental conditions.
The ab85486 concentration was determined by Bradford assay.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Chromogranin A (CgA) sometimes called chromagranin A is a glycoprotein with a molecular weight of approximately 49–52 kDa. It is highly expressed in the secretory granules of neuroendocrine cells present in tissues such as the adrenal medulla pancreas and endocrine cells of the gastrointestinal tract. CgA identified as part of a family including chromogranin B and chromogranin C functions mechanically as a precursor to various bioactive peptides. This translates into its involvement in the storage and release of hormones and peptides within vesicles.
Biological function summary
Chromogranin A assists in the regulation of secretory pathways across multiple neuroendocrine systems. It plays a role in hormone storage conversion to active peptides and regulates the exocytosis of hormones. CgA acts within a complex contributing to secretory granule biogenesis and influencing intravesicular acidity. As a regulatory component the exact physiological roles are diverse impacting cardiovascular metabolic and immunological processes.
Pathways
Chromogranin A is involved in pathways such as the catecholamine synthesis and release pathway and the serotonergic pathway. Within these pathways it interacts with proteins like secretogranin II and parathyroid hormone (PTH). These interactions modulate neurotransmitter storage and secretion across various systems. CgA's bioactive peptides also contribute to modulating physiological processes like vasoconstriction and insulin regulation linking it closely with these signaling cascades.
Chromogranin A is a known marker for neuroendocrine tumors where its levels in blood may increase due to tumor activity. It links to diseases like pheochromocytoma and carcinoid tumors where improper regulation of CgA-associated pathways occurs. Additionally its connection to parathyroid disorders through the PTH pathway illustrates its broader implication in endocrine pathophysiology. CgA serves as an invaluable diagnostic and prognostic tool in these disease states and supports the development of targeted therapies.