Abcam, ab85659, Anti-SMG9 antibody

Size: 100µL
Rabbit Polyclonal SMG9 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SMG9.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SMG9. The exact immunogen used to generate this antibody is proprietary information.Q9H0W8

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMG9 also known as suppressor with morphogenetic effect on genitalia 9 is a critical component in RNA decay machinery. It has a molecular mass of about 81 kDa. The protein is located in the cytoplasm and nucleus of cells. SMG9 expression occurs ubiquitously across various tissues suggesting its role in fundamental cellular processes. It is not tissue-specific which indicates its importance in a multitude of cell types.
Biological function summary
SMG9 plays a role in nonsense-mediated mRNA decay (NMD) a quality control mechanism that targets defective mRNA. Within this process SMG9 forms part of the SMG1 complex which is essential in recognizing and degrading mRNAs with premature stop codons. This action helps maintain the fidelity of gene expression and prevents the translation of potentially harmful truncated proteins. The function of the SMG1-SMG9 complex emphasizes its role in cellular homeostasis and regulation.
Pathways
SMG9 integrates into the NMD pathway a critical mechanism for post-transcriptional regulation of gene expression. The NMD pathway involves several proteins including UPF1 a central player in recognizing and degrading faulty mRNAs. SMG9 directly interacts with the SMG1 kinase which phosphorylates UPF1 enhancing the accuracy of the mRNA surveillance. This interaction places SMG9 at a significant junction for controlling mRNA integrity and protecting cells from abnormal proteins.
Disruptions in the function of SMG9 have been linked to various genetic disorders such as intellectual disability. Mutations in SMG9 can lead to impaired NMD function resulting in the accumulation of dysfunctional proteins that contribute to disease pathology. The altered function of SMG9 in disorders often involves interactions with other elements of the NMD pathway including UPF1 which can disrupt normal cellular activities and promote disease development. These links suggest a potential target for therapeutic interventions to mitigate effects caused by defective mRNA decay processes.