Abcam, ab85752, Anti-hSSB1 antibody

Size: 100µg
Rabbit Polyclonal hSSB1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NABP2 aa 150 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IP, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human NABP2 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9BQ15

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HSSB1 also known as OBFC2B is a single-stranded DNA-binding protein with a molecular mass of approximately 30 kDa. It is mainly expressed in the nucleus where it plays an important role in the maintenance and protection of genomic stability. The protein recognizes and binds to single-stranded DNA which is typical during DNA replication and repair processes. hSSB1 is highly conserved across different species highlighting its important function in cellular mechanisms.
Biological function summary
This protein plays a significant role in DNA damage response and repair. hSSB1 forms a complex with the INTS3 protein as part of the MRN (MRE11-RAD50-NBS1) complex to effectively respond to DNA double-strand breaks. This action is critical to initiate the appropriate signaling cascade that leads to DNA repair. The protein's expression and activity need tight regulation to ensure genome integrity and to prevent mutations.
Pathways
HSSB1 is involved in the homologous recombination repair pathway which is essential for error-free repair of DNA double-strand breaks. It interacts with BRCA1 and RAD51 which are key players in homologous recombination ensuring accurate DNA damage repair. Additionally hSSB1 participates in the ATR signaling pathway enhancing cell cycle checkpoint activation during replication stress where it collaborates with ATR and its substrates.
HSSB1 has been implicated in cancer development particularly in breast and ovarian cancers. Defects or dysregulation in hSSB1 may lead to inadequate DNA repair increasing the likelihood of mutations that contribute to carcinogenesis. Furthermore hSSB1 has connections to neurodegenerative disorders linked through its interactions with proteins like ATM. hSSB1's influence on maintaining genomic stability places it at the intersection of various pathways that affect cellular health and disease progression.