Abcam, ab86042, Anti-Collagen IV antibody [1043]

Size: 100µg
Mouse Monoclonal COL4A1 antibody. Suitable for IHC-P, IHC-Fr and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL4A1.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1043,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human,
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Native Full Length Protein corresponding to Human COL4A1.P02462

Product details:
In IHC, recognizes the extracellular – basement membranes.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result, we are pleased to offer this antibody in a purified format as of 27
October 2017. The following lots are still unpurified and still in stock as of 27
October 2017- GR275641-7, GR275641-13, and GR275641-14. Lot numbers other than GR275641-7, GR275641-13, and GR275641-14 will be purified. Please note that the dilutions may need to be adjusted accordingly. Purified antibodies have the advantage of being enriched for the fraction of immunoglobulin that specifically reacts with the target antigen and for having a reduction of serum proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-From tissue culture supernatant, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagen IV also known as COL4A1 or collagen type IV is a structural protein essential to the basement membrane in tissues. The molecular weight of collagen type 4 varies but it generally forms a high-molecular-weight complex. This protein expresses prominently in various tissues especially in the kidneys lens capsule of the eye and skin. Collagen IV comprises six different alpha chains where its distinctiveness lies in its non-fibrillar network-forming structure which serves as a supportive scaffold for tissues and contributes to tissue regeneration and cell adhesion.
Biological function summary
The highly organized network of collagen type IV plays a supreme role in maintaining the integrity and functionality of the basement membrane. It exists predominantly as part of a supramolecular complex associating with laminin nidogen and perlecan forming a multifunctional platform. This platform facilitates cellular signaling tissue elasticity and filtration functions. The basement membrane's selective permeability heavily relies on this network structure to regulate the exchange of molecules between tissues like blood vessels and epithelial sheets.
Pathways
Collagen IV contributes to several critical functions within the extracellular matrix organization and cell-matrix adhesion pathways. Specifically it interacts intimately with integrins and matrix metalloproteinases (MMPs) playing roles in angiogenesis and tissue repair. Additionally its interactions with other collagens and integrins within these pathways enhance cellular responses to mechanical stress and contribute to structural resilience in tissues.
Mutations or abnormalities in collagen IV are linked closely with hereditary conditions such as Alport syndrome and Goodpasture syndrome. These conditions often result in compromised structural integrity and function of the basement membrane particularly affecting the kidneys and lungs. Alterations in collagen IV can influence interactions with other proteins like collagen type IV-associated proteins altering normal physiological functions and leading to tissue dysfunction and disease manifestations.