Abcam, ab86095, Anti-LARG antibody

Size: 100µg
Rabbit Polyclonal LARG antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARHGEF12 aa 1350-1400.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ARHGEF12 aa 1350-1400. The exact immunogen used to generate this antibody is proprietary information.Q9NZN5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab86095 was affinity purified using an epitope specific to LARG immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LARG also known as Leukemia Associated Rho Guanine Nucleotide Exchange Factor is a RhoGEF involved in the regulation of Rho GTPases. It functions mechanically by facilitating the exchange of GDP for GTP on Rho proteins thereby activating them. LARG has a molecular mass of approximately 173 kDa. It expresses in various tissues including the brain heart and kidney highlighting its potential involvement in diverse biological processes.
Biological function summary
LARG influences cytoskeletal dynamics and cell mobility. It associates with G-protein coupled receptor signaling complexes impacting cellular responses to external stimuli. This protein also has important roles in the regulation of cell adhesion migration and proliferation. Through interactions with other proteins such as PDZ-RhoGEF and p115-RhoGEF LARG influences the organization of the actin cytoskeleton and is involved in maintaining tissue architecture.
Pathways
LARG integrates into the RhoA signaling pathway and is an important player in the regulation of cellular structure and motility. It interacts with proteins involved in the Rho GTPase cycle such as RhoA and RhoC and modulates actin cytoskeletal arrangement. LARG also participates in the Wnt signaling pathway influencing gene expression and cell behavior. Through these interactions LARG contributes to regulating processes like cell morphology and division.
LARG has connections to certain cancers and cardiovascular disorders. It interacts with proteins such as Gα13 and RhoA which have implications in the progression of these diseases. In cancer alterations in LARG expression or function may lead to enhanced cell proliferation and metastasis. In cardiovascular disorders LARG-related pathways might affect cell contraction and architecture in vascular tissues contributing to pathological states. Understanding LARG's role in these contexts could help develop targeted therapies.