Abcam, ab86219, Recombinant human Cyclophilin A protein (Active)

Size: 100µg / 500µg
Recombinant human Cyclophilin A protein (Active) is a Human Full Length protein, in the 1 to 165 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.
Key facts
Purity:>95% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Specific activity is >650 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-Hcl pH8.0 using chymotrypsin.Activity AssayPrepare 180μl assay buffer into a suitable container:Test is 120μl of 200mM Tris-HCl pH8.0, 50μl of 20nM chymotrypsin and 10μl of 100μg/ml Cyclophilin A protein.Blank is 130μl of 200mM Tris-HCl pH8.0 and 50μl of 20nM chymotrypsin.Equilibrate to 37°C and monitor the A405nm until value is constant using a spectrophotometer.Load 20μl of 5mM Suc-AAPF-pNA into plate. (460mM Lithium chloride, 3mM 2,2,2-Trifluoroethanol in DW. The suc-AAPF-pNA is first dissolved in DMSO and diluted once in 200 mM Tris-HCl (pH8.0). Finally, manufacture the final 5mM with 460mM Lithium chloride, 3mM 2,2,2-Trifluoroethanol).Add 180μl of assay buffer in 5mM Suc-AAPF-pNA.Record the absorbance A405nm for 40 minutes at 37 °C.,
Accession:P62937,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.242% Tris, 0.116% Sodium chloride, 0.0077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Product details:
MW confirme by MALDI-TOF.
Concentration determined by Bradford assay.
Endotoxin levels determined by LAL method.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle