Abcam, ab86695, Anti-VAV2 (phospho Y172) antibody

Size: 100µL
Rabbit Polyclonal VAV2 phospho Y172 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human Guanine nucleotide exchange factor VAV2 phospho Y172 conjugated to Keyhole Limpet Haemocyanin.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Guanine nucleotide exchange factor VAV2 phospho Y172 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.P52735,
Specificity:ab86695 detects Vav1, Vav2, and Vav3 in Jurkat cells treated with pervanadate. The antibody does not detect these Vavs after treatment with alkaline phosphatase.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab86695 was cross-adsorbed to unphosphorylated VAV2 (Tyr-172) peptide before affinity purification using phospho-VAV2 (Tyr-172) peptide (without carrier)., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Stable for 12 months at -20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VAV2 also known as Vav 2 guanine nucleotide exchange factor is a member of the VAV family of proteins which function as guanine nucleotide exchange factors (GEFs). It has a molecular mass of approximately 100 kDa. The protein converts inactive GDP-bound Rho family GTPases such as Rac1 and Cdc42 into their active GTP-bound form. VAV2 is expressed in various tissues including the brain heart and lung indicating its broad role in cellular signaling across different systems.
Biological function summary
VAV2 influences multiple cellular processes through its role as a GEF. This protein does not form a part of a static complex but interacts dynamically with other signaling components. VAV2 regulates cytoskeleton organization cell migration and growth by activating Rho family GTPases. Its activity impacts processes such as neuronal development and immune response by modifying the actin cytoskeleton which is important for cell movement and morphological changes.
Pathways
VAV2 plays an integral role in signaling cascades like the Ras and MAPK pathways. It interacts closely with proteins like Rac1 and Cdc42 to propagate signals that influence cell proliferation and survival. By facilitating the switch from inactive GDP-bound states to active GTP-bound forms of Rho GTPases VAV2 integrates signals from cell surface receptors to downstream effectors that mediate cellular responses to external stimuli.
VAV2’s function connects to cancer progression and cardiovascular disorders. Changes in VAV2 expression or activity can lead to abnormal cell growth and differentiation linking it to cancerous transformations. Additionally because VAV2 modulates cell migration and cytoskeletal dynamics its malfunction can contribute to cardiovascular diseases by affecting vascular smooth muscle cells and endothelial cells. In both contexts VAV2's interactions with Rho family proteins like Rac1 highlights its influence in disease mechanisms.