Abcam, ab86734, Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3]

Size: 100µL
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) is a mouse recombinant multiclonal antibody detecting pan Cytokeratin in Flow Cytometry, IHC-P, ICC/IF . Suitable for Chicken, Dog, Human, Pig . - Recombinant format for unrivalled batch-batch consistency - Over 60 publications - Trusted since 2010
Key facts
Host species:Mouse,
Clonality:Multiclonal,
Clone number:AE1/AE3 + 5D3,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human, Chicken, Pig, Goat, Dog,
Applications:Flow Cyt, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Full Length Protein corresponding to Human KRT8. The exact immunogen used to generate this antibody is proprietary information.P05787,
Specificity:The ab86734 is a combination of [AE1/AE3] and [5D3] clones and can be used to detect most human epithelia. [AE1/AE3] recognizes acidic and basic subfamilies of cytokeratins, with molecular weights ranging from 40 to 67 kDa. [5D3] recognizes Cytokeratin 8 and 18 intermediate filament proteins. In normal tissues, [5D3] recognizes all simple and glandular epithelium. It has been observed that [AE1/AE3] has had problems marking certain tissues types and adenocarcinomas. The addition of [5D3] may remedy some of the limitations observed when staining with [AE1/AE3] alone.

Product details:
Product Specifications
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) is a recombinant mouse multiclonal antibody which is comprised of multiple monoclonal antibodies targeting different epitopes and recapitulating the performance of a polyclonal.
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) is validated for use in Flow Cyt, ICC/IF and IHC-P in chicken, dog, human and pig samples.
Anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) specifically detects pan Cytokeratin/ Keratin, type II cytoskeletal 1b (UniProt ID: Q7Z794; Molecular weight: 62kDa) and is sold in a 100 µL selling size.
Quality and Validation
Abcams high quality manufacturing and validation processes ensure anti-pan Cytokeratin antibody [AE1/AE3 + 5D3] (ab86734) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A/G, Storage buffer-Preservative: 0.09% Sodium azide, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pan cytokeratin also known as cytokeratin AE1/AE3 is a cytoskeletal protein complex that consists of different keratin polypeptides. It mechanically supports epithelial cells contributing to their structural integrity. The protein mass is variable due to its composite nature ranging between 40 to 67 kDa. Pan cytokeratin is expressed in epithelial tissues throughout the body serving as a reliable marker for identifying epithelial origin cells in histological studies.
Biological function summary
Keratins in pan cytokeratin contribute to maintaining structural stability within epithelial cells. They act as components of the cytoskeletal network interacting with other cellular structures to provide protection from stress and mechanical damage. Pan cytokeratin exists as part of the intermediate filament protein complex which plays a significant role in cellular mechanics and cohesion essential for tissue integrity.
Pathways
Intermediate filaments containing pan cytokeratin participate in pathways related to cell differentiation and stress response. Its interactions with other proteins like desmoplakin and plectin link the cytoskeletal filaments to cell membranes and the extracellular matrix. This involvement is important in pathways related to cellular structural integrity and epithelial-mesenchymal transition (EMT).
Pan cytokeratin serves as a critical marker in the diagnosis of epithelial-derived cancers such as carcinoma and conditions like psoriasis. Abnormal expression patterns of pan cytokeratin indicate tumor presence and origin. Its connection to proteins such as E-cadherin and vimentin assists in understanding the progression and metastasis of cancer by facilitating EMT and tissue invasion diagnostics.