Abcam, ab87199, Anti-Muscarinic Acetylcholine Receptor M3/CHRM3 antibody

Size: 100µg
Rabbit Polyclonal Muscarinic Acetylcholine Receptor M3/CHRM3 antibody. Suitable for WB, IHC-P and reacts with Rat samples. Cited in 13 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Muscarinic Acetylcholine Receptor M3 also known as CHRM3 or M3 receptor plays an important role in various physiological processes. This receptor is part of the G-protein coupled receptor family and binds acetylcholine. The mass of CHRM3 is around 65 kDa. CHRM3 is widely expressed in various tissues including smooth muscle exocrine glands and in some cases the central nervous system. This extensive expression indicates its significance in multiple bodily functions.
Biological function summary
CHRM3 mediates cellular responses such as smooth muscle contraction and glandular secretion. It operates as part of a larger receptor complex that facilitates the signal transduction process. When acetylcholine binds to CHRM3 it activates downstream signaling pathways involving various intracellular messengers. This activation influences activities such as bronchoconstriction and salivation emphasizing the importance of M3 receptor in autonomic nervous system regulation.
Pathways
Studies show that CHRM3 engages with the inositol phospholipid-calcium signaling pathway. This pathway mobilizes calcium from intracellular stores working together with proteins like G-proteins and protein kinase C. These interactions lead to physiological effects such as muscle contraction and secretion. Moreover CHRM3 links with the MAPK/ERK pathway conveying signals that influence cell growth and differentiation. The intertwining of these pathways highlights CHRM3’s role in regulating key cellular functions.
CHRM3’s malfunction associates with asthma and chronic obstructive pulmonary disease (COPD). Dysregulated CHRM3 signaling can exacerbate symptoms involving bronchoconstriction and mucus secretion. Furthermore CHRM3 connects to proteins affecting airway smooth muscle cells intensifying disease conditions. Its relevance in exocrine gland regulation also implicates CHRM3 in conditions like Sjögren's syndrome where it may impact salivary and lacrimal gland secretion. Understanding these connections aids in developing targeted therapies for these ailments.