Abcam, ab87539, Anti-SMG6 antibody

Size: 100µg
Rabbit Polyclonal SMG6 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 30 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human, Mouse,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMG6 also known as Suppressor with Morphological Effect on Genitalia 6 is an important component in the mRNA surveillance machinery and plays an important role in nonsense-mediated mRNA decay (NMD). It is a protein with a molecular mass of approximately 180 kDa. SMG6 exhibits endonuclease activity performing its function by cleaving mRNA substrates that contain premature stop codons. The protein is ubiquitously expressed across various tissues indicating its fundamental role in maintaining cellular functions.
Biological function summary
SMG6 associates with the core NMD factor UPF1 forming part of a complex involved in degrading faulty mRNA transcripts. This degradation process ensures that aberrant mRNAs do not translate into dysfunctional proteins essential for maintaining proteome integrity. SMG6 through its endonuclease activity precisely cleaves the target mRNA facilitating its decay and recycling safeguarding cellular homeostasis.
Pathways
SMG6 functions primarily within the nonsense-mediated decay (NMD) pathway a critical cellular quality control mechanism. This pathway intersects with other cellular processes such as mRNA metabolism and translation regulation. The protein interacts closely with both UPF1 and SMG7 which are essential proteins for efficient NMD. SMG6 ensures the NMD pathway operates effectively thereby maintaining cellular equilibrium against aberrant genetic events.
Aberrations in SMG6 function link to cancer and neurodegenerative disorders. Mutations or dysregulation of SMG6 may lead to an inability to eliminate faulty mRNAs contributing to the accumulation of defective proteins that play roles in tumorigenesis or neurodegeneration. In cancer altered interactions between SMG6 and proteins like UPF1 can result in unchecked cell proliferation. Similarly disruptions in SMG6-associated pathways might lead to neurodegenerative processes by allowing the accumulation of toxic protein products in neuronal tissues.