Abcam, ab89016, Anti-EIF5B antibody

Size: 50µg
Mouse Polyclonal EIF5B antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human EIF5B.
Key facts
Host species:Mouse,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human EIF5B.O60841

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EIF5B also known as eukaryotic translation initiation factor 5B plays a significant mechanical role in the initiation of mRNA translation. This protein has a molecular weight of approximately 140 kDa. EIF5B is highly conserved across eukaryotes and is found in the cytoplasm where its expression facilitates the assembly of the 80S ribosome. The protein interacts with the ribosomal subunits and GTP an energy-carrying molecule to promote the joining of the 40S and 60S subunits forming a functional unit ready for protein synthesis.
Biological function summary
EIF5B is important in facilitating vectorial movement during translation initiation. It plays a role in the ribosomal subunit assembly and interacts with several initiation factors and ribosomal proteins. EIF5B does not work alone; instead it is part of a complex that ensures the proper alignment and initiation of translation. By coordinating these interactions EIF5B ensures that protein synthesis starts at the correct part of the mRNA template.
Pathways
EIF5B is deeply involved in the translation initiation and protein synthesis pathways. The protein interacts with other initiation factors like eIF1A ensuring the fidelity of start codon recognition and subunit joining. EIF5B also associates with pathways related to ribosome biogenesis. These interactions position EIF5B as a link between the assembly of ribosomal machinery and the broader cellular pathways controlling protein synthesis.
EIF5B has connections to several pathologies particularly cancer and neurodegenerative diseases. Its role in ensuring accurate translation initiation makes aberrations in EIF5B linked to unregulated cell growth seen in cancers. In neurodegenerative disorders improper translation regulation can contribute to dysfunctional protein accumulation. Additional proteins such as eIF2B and eIF4E may also play interconnected roles in these diseases with a network of interactions influencing cellular outcomes in disease contexts.