Abcam, ab8933, Anti-pan-AKT (phospho T308) antibody

Size: 100µg
Rabbit Polyclonal AKT1 phospho T308 antibody. Suitable for Dot, WB, IHC-P, ELISA and reacts with Recombinant full length protein, Human, Recombinant full length protein - Human samples. Cited in 102 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ELISA, WB, Dot, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody was made against a peptide directed against the phosphorylated form of AKT1 at T308, but due to a high degree of homology it is predicted to cross react with AKT2 and AKT3 if they are phosphorylated at the corresponding residue. Weak cross reactivity with AKT2.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-AKT phospho T308 Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities., Storage buffer-pH: 7.2Preservative: 0.01% Sodium azideConstituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target known as pan-AKT or AKT pan is a serine/threonine kinase involved in several cellular processes. It is also known by the names AKT1 AKT2 and AKT3 reflecting the different isoforms of the AKT protein. This enzyme is approximately 56 kDa in molecular weight although this may slightly vary depending on the isoform. AKT is widely expressed in many tissues including muscles fat and the brain. Scientists often detect the protein via AKT western blot reconfirming its detailed molecular weight and AKT protein size in different tissue samples.
Biological function summary
The AKT protein plays a significant role in cellular signaling regulating cell growth survival and metabolism. The AKT protein does not function alone; it interacts with various partners and is part of the larger signaling complexes. AKT activation requires binding to phosphatidylinositol (345)-trisphosphate a process which is dependent on phosphoinositide 3-kinase (PI3K). This activation leads to downstream effects that are vital for proper cellular functions such as nutrient uptake and glucose metabolism.
Pathways
AKT is an important player in the PI3K/AKT/mTOR pathway one of the most important signaling pathways controlling cell growth proliferation and survival. Within this pathway AKT interacts with many proteins including mTOR and PTEN helping to mediate its diverse biological effects. Additionally the AKT signaling pathway has links to the insulin signaling pathway where it influences the activity of several key metabolic regulators.
AKT activity has connections to cancer and diabetes. Abnormal AKT activation is a feature of many cancers as it can lead to uncontrolled cell growth and survival. In cancer AKT often works alongside other proteins like p53 which are important in tumor suppression and cell cycle regulation. In diabetes altered AKT signaling affects insulin sensitivity as seen with proteins such as IRS1 that interact with the insulin pathway. Understanding AKT's role in these diseases continues to be a focus for developing targeted therapies.