Abcam, ab92670, Anti-RFX3 antibody

Size: 100µg
Rabbit Polyclonal RFX3 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RFX3.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human RFX3. The exact immunogen used to generate this antibody is proprietary information.P48380

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The RFX3 protein also known as Regulatory Factor X 3 plays a significant role in gene transcription regulation. It has a molecular mass of approximately 83 kDa. RFX3 functions as a transcription factor binding specifically to X-box motifs within DNA influencing the expression of various genes important for cell processes. This protein is present in a variety of tissues including the brain kidney and pancreas suggesting its diverse and widespread role in cellular activities.
Biological function summary
The function of RFX3 involves the modulation of genes critical for cilia development and function. RFX3 forms part of a gene regulatory complex that oversees ciliogenesis. Through this complex it controls the transcription of genes essential for the formation of motile and primary cilia. The activity of RFX3 maintains proper cilia structure and movement essential for cellular signaling and environmental sensing.
Pathways
The involvement of RFX3 focuses largely on the ciliogenesis and hedgehog signaling pathways. RFX3 regulates genes necessary for the assembly and function of cilia linking it to the hedgehog signaling pathway where cilia play an important role in signal reception and transduction. RFX3 interacts with proteins like RFX1 and RFX2 which share similar DNA-binding domains highlighting its preserved function across these pathways.
Disruptions in RFX3 can relate to ciliopathies and diabetes. Ciliopathies such as Joubert syndrome arise from defective ciliogenesis due to compromised RFX3 function with related proteins like RFX1 also being implicated. Diabetes connects to RFX3 through its expression in pancreatic beta cells suggesting a role in insulin regulation and glucose metabolism. Changes in RFX3 levels or function can affect genes involved in these conditions indicating its importance in maintaining normal physiological processes.