Abcam, ab9324, Anti-IL-6 antibody [1.2-2B11-2G10]

Size: 500µg
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) is a mouse monoclonal antibody detecting IL-6 in Western Blot, IHC-P, ELISA . Suitable for Human, Rat . - Over 320 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1.2-2B11-2G10,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Rat, Human,
Applications:sELISA, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human IL6.P05231

Product details:
Product Specifications
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) is a mouse monoclonal antibody and is validated for use in IHC-P, WB, sELISA in human, rat samples.
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) specifically detects IL-6 (UniProt ID: P05231; Molecular weight: 21kDa) and is sold in 500 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) has high sensitivity and specificity.
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) has been cited over 320 times in peer reviewed journals and is trusted by the scientific community.
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) has 20 independent reviews from customers.
Although some customers have been successful in IHC we no longer batch test in this application.

Properties and Storage Information:
Form-Lyophilized, Reconstitution-Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary., Purification technique-Affinity purification Protein A, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.
Biological function summary
IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.
Pathways
IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.
IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.