Abcam, ab93926, Anti-GSK3 beta antibody [3D10]

Size: 100µL
Anti-GSK3 beta antibody [3D10] (ab93926) is a mouse monoclonal antibody detecting GSK3 beta in Western Blot, Flow Cytometry, IHC-P, ICC/IF . Suitable for Human, Mouse, Rat . - Over 150 publications - Trusted since 2010
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3D10,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GSK3B. The exact immunogen used to generate this antibody is proprietary information.P49841

Product details:
What is this antibody validated in?
Anti-GSK3 beta antibody [3D10] (ab93926) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of GSK3 beta?
Anti-GSK3 beta [3D10] (ab93926) specifically detects a band for GSK3 beta (UniProt: P49841) at a molecular weight of 47kDa.
Trusted by the scientific community
Anti-GSK3 beta [3D10] (ab93926) was first used in a scientific publication in 2010 and has been cited over 150 times in peer-reviewed journals.
Reviewed by scientists
Anti-GSK3 beta [3D10] (ab93926) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glycogen synthase kinase 3 beta or GSK3 beta is a serine/threonine protein kinase that regulates various cellular processes. It is also referred to as GSK3B and has a molecular weight of approximately 47 kilodaltons (kDa). This enzyme is widely expressed in many tissues with notably high expression levels in the brain. Mechanically GSK3 beta phosphorylates target proteins affecting their activity stability and interactions with other proteins.
Biological function summary
As an important regulatory protein GSK3 beta plays a role in multiple cellular functions including metabolism cell cycle and cell signaling. It often interacts with components in signaling complexes and modulates the activity of these complexes involving phosphorylation. It is involved in the regulation of transcription factors such as c-Myc and Β-catenin influencing cell fate and survival. This ability to modulate diverse functions highlights GSK3 beta's importance in cellular regulation.
Pathways
GSK3 beta participates in significant signaling pathways such as the Wnt/Β-catenin and insulin signaling pathways. In the Wnt pathway GSK3 beta phosphorylates Β-catenin marking it for degradation which regulates gene transcription. In insulin signaling it interacts with proteins like AKT to mediate glycogen synthesis. These interactions highlight its role in maintaining cellular homeostasis and energy balance.
GSK3 beta has implications in Alzheimer's disease and type 2 diabetes. It is associated with tau protein hyperphosphorylation and neurofibrillary tangles in Alzheimer's disease. In diabetes its role in insulin signaling impacts glucose metabolism. Further GSK3 beta's dysfunction relates to disrupted signaling related to these conditions positioning it as a potential therapeutic target.