Abcam, ab94244, RYBP overexpression 293T lysate (whole cell)

Size: 100µL
RYBP overexpression 293T lysate (whole cell) suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
Key facts
Species or organism:Human,
Form:LiquidSee storage information

Product details:
ab94244 is a 293T cell transfected lysate in which Human DEDAF has been transiently over-expressed using a pCMV-DEDAF full length plasmid. The lysate is provided in 1X Sample Buffer.

Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RYBP also known as Ring1 and YY1 Binding Protein has a critical mechanical role in gene regulation. It has a molecular weight of approximately 28 kDa. It is a part of the Polycomb group (PcG) proteins which take part in chromatin remodeling to regulate gene expression. RYBP is found in the nucleus of cells and is known to bind DNA directly. Its expression occurs widely across various tissues indicating its broad involvement in cellular functions.
Biological function summary
This protein actively participates in maintaining transcriptional repression through its involvement in the Polycomb Repressive Complex 1 (PRC1). PRC1 includes other key proteins such as Ring1A/B and CBX proteins which work together to modulate chromatin structure. RYBP contributes to the ubiquitination of histone H2A a process essential for maintaining gene silencing. This activity is important during developmental stages influencing cell fate decisions and lineage commitment.
Pathways
RYBP plays an essential role in the regulation of the Wnt signaling pathway and the apoptosis pathway. Within the Wnt pathway RYBP acts in part by interacting with proteins such as β-catenin influencing cell proliferation and differentiation. In the apoptosis pathway it is involved through its interactions with E3 ubiquitin ligases potentially affecting cell death regulation. These pathways showcase how RYBP integrates into important cellular processes impacting cellular homeostasis.
RYBP has connections to cancer and neurodevelopmental disorders. Its altered expression is linked to tumor progression where it interacts with other PcG proteins like EZH2 often showing overexpression in cancers. These interactions can lead to abnormal gene silencing contributing to oncogenesis. In neurodevelopmental contexts disruptions in RYBP's function are associated with changes in neural differentiation and might correlate with disorders like autism spectrum disorders highlighting its broader relevance beyond typical gene expression roles.