Abcam, ab9440, Anti-L Glutamate antibody

Size: 500µL
Rabbit Polyclonal L Glutamate antibody. Suitable for IHC-Fr, IHC-FoFr and reacts with Chemical samples. Cited in 16 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to L Glutamate.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Applications:IHC-Fr, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:The antibody is calibrated against a spectrum of antigens to assure hapten selectivity. No measurable cross-reactivity (<1:1000) was detected against glutamate in peptides or proteins. Fixed tissue cross-reactivity was tested with known targets at the recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) was detected against L-alanine, gamma-aminobutyrate, D/L-aspartate, agmatine, guanidine, D/L-arginine, L-citrulline, L-cysteine, D/L-glutamine, glutathione, glycine, L-lysine, L-ornithine, L-serine, taurine, L-threonine, L-tryptophan, L-tyrosine. Modest cross-reactivity (1:20) was detected against D-glutamate. Significant cross-reactivity (1:8) was detected against free NAAG in competition assays (NAAG is not retained in aldehyde-fixed tissue).

Product details:
The product is optimized for HPI/EHPI with gold or fluorescence detection using etched plastic sections. Filter diluted reagents with 0.2 mm syringe filters before use on EM grids. Enzyme-linked visualizations can be used but will compress the signal dynamic range and are less sensitive.

Properties and Storage Information:
Form-Liquid, Purity-IgG fraction, Storage buffer-Preservative: 0.05% Thimerosal (merthiolate)Constituents: Phosphate Buffer, 1% Goat Serum, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
L-glutamate also called glutamic acid is an important neurotransmitter in the central nervous system. This small amino acid has a molecular weight of 147.13 g/mol. Neurons and glial cells both express L-glutamate which allows it to function widely in the brain. Synaptic terminals release L-glutamate into the synaptic cleft where it binds to glutamate receptors on the postsynaptic neuron to propagate neural signals.
Biological function summary
L-glutamate serves key roles in synaptic plasticity and cognitive functions including learning and memory. It acts as the main excitatory neurotransmitter in the human brain and is essential for normal brain activities. L-glutamate does not belong to a protein complex but it operates closely with other neurotransmitters and their receptors ensuring proper signal transmission between neurons.
Pathways
L-glutamate is involved in synaptic signaling and metabolic pathways like the glutamatergic synapse pathway. This amino acid interacts with proteins such as NMDA and AMPA receptors that are integral to these pathways. The proper functioning of these pathways is critical for maintaining excitatory neurotransmission and overall brain health.
Irregular L-glutamate signaling relates to conditions such as Alzheimer’s disease and epilepsy. In Alzheimer’s disease altered glutamate signaling contributes to neurodegeneration with NMDA receptor overactivation playing a role. In epilepsy excessive glutamate release leads to hyperexcitation contributing to seizures. Understanding these associations helps to identify potential therapeutic targets for treating these disorders.