Abcam, ab9512, Anti-Aquaporin 4 antibody [4/18]

Size: 100µg
Anti-Aquaporin 4 antibody [4/18] (ab9512) is a mouse monoclonal antibody detecting Aquaporin 4 in IHC-P, IHC-Fr, ELISA . Suitable for Human, Mouse, Rabbit, Rat, Zebrafish . - Over 40 publications - Trusted since 2002
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:4/18,
Isotype:IgG3,
Light chain type:kappa,
Carrier free:No,
Reacts with:Rat, Zebrafish, Mouse, Rabbit, Human,
Applications:IHC-P, IHC-Fr, ELISA, IHC-FoFrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Rat Aqp4 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.P47863,
Epitope:Intracellular C-terminal AQP 4 epitope

Product details:
What is this antibody validated in?
Anti-Aquaporin 4 antibody [4/18] (ab9512) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), ELISA in Human, Mouse, Rabbit, Rat, Zebrafish samples.
Trusted by the scientific community
Anti-Aquaporin 4 [4/18] (ab9512) was first used in a scientific publication in 2002 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Aquaporin 4 [4/18] (ab9512) has over 5 independent reviews from customers.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.097% Sodium azideConstituents: PBS, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aquaporin 4 also known as AQP4 functions as a water channel protein that facilitates the transport of water across cell membranes. It is composed of approximately 323 amino acids resulting in a molecular mass of around 34-38 kDa. This target expresses most in the central nervous system especially in the astrocytes of the brain and spinal cord. Other expression sites include skeletal muscle kidneys and lungs. AQP4 is often studied using various tools including anti-aquaporin 4 antibodies and specific reagents like AQP4 Ab and anti-AQP4.
Biological function summary
Water balance and neural activity regulation are significantly influenced by aquaporin 4. This protein forms a part of the orthogonal array of particles (OAPs) in the plasma membrane which enhances its water permeability function. Through its concentration at the blood-brain barrier AQP4 contributes to maintaining brain homeostasis by regulating osmotic gradients and extracellular space ion concentrations. Its expression and activity may affect critical processes like neuroexcitation and neuroprotection.
Pathways
Aquaporin 4 actively engages in critical water transport and blood-brain barrier integrity pathways. The passive yet selective water transport facilitated by AQP4 impacts pathways including the transcellular pathway that helps in maintaining the homeostatic and osmotic balance important for brain function. Furthermore AQP4 acts in relation to other proteins such as aquaporin 1 and connexin 43 indicating its role in gap junction communication within the brain.
Aquaporin 4 is intricately linked to conditions like neuromyelitis optica (NMO) and cerebral edema. In NMO autoantibodies such as anti-AQP4 target the protein causing demyelination and inflammation of the optic nerve and spinal cord. In cases of cerebral edema an imbalance in AQP4 expression can disrupt water homeostasis leading to exacerbated swelling. AQP4's interaction with proteins like myelin basic protein in these diseases underlines its significance in neurodegeneration and inflammatory processes.