Abcam, ab95949, Anti-UAP1 antibody

Size: 50µL
Rabbit Polyclonal UAP1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human UAP1 aa 50-500.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human UAP1 aa 50-500. The exact immunogen used to generate this antibody is proprietary information.Q16222

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UDP-N-acetylglucosamine pyrophosphorylase also known as UAP1 is an enzyme that catalyzes the formation of uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) from UTP and N-acetylglucosamine-1-phosphate. UAP1 has a mass of approximately 56 kDa. This enzyme is expressed in various tissues but shows higher expression levels in skeletal muscle heart and liver. In these tissues UAP1 plays a significant role in synthesizing nucleotides and certain sugars that are essential for other cellular processes.
Biological function summary
The role of UAP1 is central to the biosynthetic pathway of complex carbohydrates. UAP1 participates in the synthesis of UDP-GlcNAc an essential precursor for glycoproteins and glycosaminoglycans. This function positions UAP1 as a component of a larger amino sugar complex involved in glycosylation which is critical for protein folding and stability. By producing UDP-GlcNAc UAP1 influences various cellular functions including signaling and metabolism.
Pathways
UAP1 lies at the intersection of the hexosamine biosynthetic pathway and glycosylation processes. It provides the substrate necessary for O-GlcNAcylation a modification that impacts protein function and regulation. This pathway links UAP1 to other proteins such as O-GlcNAc transferase (OGT) which modifies serine and threonine residues of many proteins. Together they affect cellular activities like nutrient sensing and transcription.
Altered UAP1 function links to metabolic and degenerative conditions. Abnormal UAP1 activity associates with type 2 diabetes due to its role in the hexosamine biosynthetic pathway that modulates insulin signaling. Furthermore its involvement in glycosylation pathways connects UAP1 to congenital disorders of glycosylation (CDG) where deficient glycosylation leads to multiorgan dysfunction. Changes in UAP1 expression or function can impair glycosylation potentially leading to dysregulation of related proteins such as insulin receptor substrates in diabetes or enzymes impacted in CDG.