Abcam, ab96084, Anti-NT5C2 antibody

Size: 50µL
Rabbit Polyclonal NT5C2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NT5C2 aa 100-350.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human NT5C2 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.P49902

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NT5C2 protein also known as cytosolic 5'-nucleotidase II plays an important role in nucleotide metabolism. It has a molecular mass of approximately 65 kDa. Researchers observe its expression in various tissues with higher levels typically found in the liver heart and brain. NT5C2 controls the conversion of nucleoside monophosphates to nucleosides and inorganic phosphate acting to regulate intracellular nucleotide levels.
Biological function summary
NT5C2 influences purine metabolism specifically in the catabolism of nucleotides. It does not function as part of a larger protein complex. Instead NT5C2 plays its role independently within the cell. This enzymatic activity contributes to maintaining nucleotide balance essential for DNA and RNA synthesis and repair and affects overall cellular metabolism.
Pathways
NT5C2 appears important in purine and pyrimidine metabolism pathways. Within these it directly impacts salvage pathways where it catalyzes reactions that recycle nucleotides from nucleic acid degradation. Its activity is relevant to proteins like APRT and HPRT which also participate in purine salvage processes ensuring efficient nucleotide utilization.
NT5C2 has associations with certain leukemias and neurological conditions. Mutations and dysregulation in NT5C2 can lead to chemoresistance in acute lymphoblastic leukemia affecting treatment efficacy. Additionally its connection with purine metabolism links it to Lesch-Nyhan syndrome where dysfunctional purine salvage causes severe neurological and behavioral abnormalities. The interaction with proteins like HPRT in Lesch-Nyhan syndrome accentuates the metabolic disruption observed.