Abcam, ab9642, Recombinant human TNF alpha protein

Size: 10µg / 50µg
Recombinant human TNF alpha protein is a Human Full Length protein, in the 77 to 233 aa range with >98% purity, < 1 EU/µg endotoxin level and suitable for ELISA, SDS-PAGE, Functional studies and HPLC. The predicted molecular weight of ab9642 protein is 17.4 kDa. - Save time and ensure accurate results - use our TNF alpha protein as a control - Optimal protein bioactivity and stability - Available in different sizes to fit your experimental needs - Cited in over 25 publications
Key facts
Purity:>98% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:FuncS, SDS-PAGE, HPLC, sELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The ED50, as determined by the cytolysis of murine L929 cells in the presence of Actinomyocin D, is ≤ 0.05 ng/mL, corresponding to a specific activity of ≥ 2 x 107 units/mg.,
Accession:P01375,
Animal free:No,
Carrier free:Yes,
Species:Human,
Reconstitution:Reconstitute in water,
Storage buffer:Constituents: 0.12% Sodium chloride, 0.049% Sodium phosphate

Product details:
Ensure the validity of your result using our bioactive recombinant human TNF alpha protein ab9642 as a positive control in SDS-PAGE and HPLC. The rTNF-alpha has a molecular weight of 17.4 kDa and corresponds to the C-terminal extracellular domain of the full length transmembrane protein.
Analyze your ELISA data using the rTNF alpha ab9642 protein to generate and plot the standard curve.
Check out our protein gel staining guide for SDS-PAGE
Check out our ELISA protocol for more information

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle