Abcam, ab96474, Anti-HuD antibody

Size: 100µg
Rabbit Polyclonal HuD antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The HuD protein also known as ELAV-like protein 4 is a neuronal RNA-binding protein with a molecular mass of approximately 40 kDa. It belongs to the ELAV family of proteins which is well-studied for its involvement in mRNA processing. HuD expresses predominantly in the nervous system where it is present in neurons and developing neural tissue. The protein localizes to the cytoplasm where it binds to mRNA influencing the stability and translation of target mRNAs.
Biological function summary
HuD is essential in regulating neuronal differentiation and function. The protein binds to adenylate-uridylate-rich elements (AREs) within the 3' untranslated regions (UTR) of specific mRNAs. It stabilizes these mRNAs contributing to the post-transcriptional regulation of genes linked to synaptic plasticity and neuronal development. HuD does not typically form part of a larger complex but interacts with other RNA-binding proteins to exert its regulatory effects.
Pathways
HuD plays a significant role in neuronal development and synaptic plasticity. It involves in pathways like the MAPK signaling pathway which is critical for cell differentiation and the mTOR pathway which modulates protein synthesis. In these pathways HuD interacts with proteins such as CPEB and FMRP which also have functions related to RNA regulation and neuronal growth.
HuD's function impacts neurological conditions such as Alzheimer's disease and certain types of cancer. Changes in the expression or activity of HuD can influence the progression of Alzheimer's disease by affecting mRNA stability of genes associated with neural health. Additionally abnormal HuD expression links to neuroblastoma a cancer showing high HuD levels promoting uncontrolled cell growth. In these diseases HuD interacts with tau protein and MYCN respectively contributing to disease mechanisms.