Abcam, ab97303, Anti-RNF114 antibody

Size: 50µL
Rabbit Polyclonal RNF114 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF114 aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RNF114 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q9Y508

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF114 also known as zinc finger protein 313 is a RING-type E3 ubiquitin ligase with a mass of approximately 51 kDa. It plays a mechanical role in tagging proteins for ubiquitination marking them for degradation by the proteasome. RNF114 is expressed mainly in the skin but can also be found in immune cells such as T-cells and macrophages. Its E3 ubiquitin ligase activity is important for regulating protein stability and cellular processes.
Biological function summary
RNF114 has a significant role in regulating immune response and cell proliferation. It participates in pathways that control inflammation by modulating the activity of NF-kB an important transcription factor in immune response. RNF114 also influences cell cycle regulation through its interactions with other proteins. While it does not form a strict complex it interacts closely with substrates it ubiquitinates to affect signaling pathways critical for cellular responses.
Pathways
Multiple cellular processes engage RNF114. It plays a part in the NF-kB signaling pathway and the Wnt signaling pathway. In the NF-kB signaling pathway RNF114 helps control the stability of specific proteins maintaining a balance in immune responses. In the Wnt pathway it has been indicated to interact with proteins like β-catenin influencing gene expression that governs cell growth and differentiation both important in development and immune function.
A few studies connect RNF114 with autoimmune diseases and cancer. For example its dysregulation has associations with psoriasis an immune-mediated disease leading to excessive skin cell proliferation. Dysregulation of RNF114 has also been noted in certain cancer types possibly through interactions with proteins such as tumor suppressors affecting pathways regulating cell growth and death. Understanding these interactions can provide insights into therapeutic targets for these conditions.