Abcam, ab97520, Anti-SAFB antibody

Size: 50µL
Rabbit Polyclonal SAFB antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SAFB aa 450-750.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SAFB aa 450-750. The exact immunogen used to generate this antibody is proprietary information.Q15424

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SAFB also known as Scaffold Attachment Factor B is a protein that participates in multiple cellular functions. It has a molecular weight of approximately 135 kDa. Researchers observe its expression in the nucleus of a variety of cell types including epithelial and fibroblast cells. In a mechanical context SAFB plays a role in binding to scaffold/matrix-attachment regions (S/MARs) of DNA influencing transcription and chromatin organization.
Biological function summary
SAFB works as a multifunctional protein that regulates gene expression and RNA processing. It acts as part of protein complexes such as those involved in modulating estrogen receptor activity. SAFB interacts with chromatin and RNA acting as a bridge between these important cellular components. It influences transcription by functioning as both a transcriptional repressor and activator depending on the context within the cell.
Pathways
Several cellular interactions highlight the role of SAFB within nuclear signaling networks like the estrogen signaling pathway and stress response pathways. SAFB associates with proteins such as estrogen receptor α (ERα) and heat shock proteins (HSP) in these pathways. Its interactions help modulate dynamics within these networks impacting cellular responses to hormonal and stress stimuli.
Researchers have connected SAFB with breast cancer and hormone-related conditions. In breast cancer SAFB's interaction with ERα suggests a role in cancer cell proliferation and response to hormonal therapy. Connections also exist with stress-response proteins like HSP70 indicating potential involvement in the cellular stress framework. Such relationships point towards SAFB's roles in disease progression and possible therapeutic targeting.