Abcam, ab97785, Anti-FGD4 antibody

Size: 50µL
Rabbit Polyclonal FGD4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FGD4 aa 1-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FGD4 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.Q96M96

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7Preservative: 0.01% Thimerosal (merthiolate)Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FGD4 also known as Frabin acts as a guanine nucleotide exchange factor (GEF) that specifically activates Cdc42 a small GTPase. The FGD4 protein typically has a molecular mass of approximately 67 kDa. It is mainly expressed in tissues such as peripheral nerve brain and kidney. The protein contains several domains including a Dbl homology (DH) domain and a pleckstrin homology (PH) domain which are instrumental in its GEF activity.
Biological function summary
The FGD4 protein is essential in regulating the actin cytoskeleton dynamics and cell shape changes. FGD4 actively participates in cellular processes like migration invasion and polarization by facilitating actin filament assembly. Although not part of a larger protein complex its function in the actin cytoskeleton impacts various downstream signaling pathways influencing cell movement and morphology.
Pathways
FGD4 plays a significant role in the Rho GTPase cycle which regulates actin cytoskeleton remodeling. Within this pathway FGD4 activates Cdc42 leading to interactions with proteins like N-WASP that mediate actin polymerization through the Arp2/3 complex. Additionally FGD4 has associations with the PI3K/Akt signaling pathway influencing cell growth and survival decisions linking indirectly with proteins like PAK1 and PI3K.
FGD4 mutations have a known association with Charcot-Marie-Tooth disease type 4H a hereditary neurological disorder. The protein's disruption affects peripheral nerve function showing a direct link to demyelinating processes. Moreover connections exist between FGD4 dysregulation and certain cancer types such as breast cancer where aberrant expression may contribute to invasive cell behavior. In these contexts FGD4 interacts with factors like Cdc42 and Rac1 reflecting its role in cytoskeletal and cellular dynamics essential for disease progression.