Abcam, ab99352, Anti-Anillin antibody

Size: 100µL
Rabbit Polyclonal Anillin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ANLN aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human ANLN aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q9NQW6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Anillin also known as ANLN is a conserved protein with a molecular weight of approximately 124 kDa. It plays an important role in the regulation of the cytoskeleton particularly during cell division. Anillin localizes mainly at the cleavage furrow during cytokinesis and interacts with several cytoskeletal components including actin filaments and myosin. Expression of Anillin occurs in various tissues but it is most abundant in rapidly dividing cells such as those found in cancers and during embryonic development.
Biological function summary
Anillin acts as a scaffold protein coordinating the assembly of the contractile ring during mitosis and cytokinesis. By binding to actin and myosin and sometimes septins Anillin ensures the successful completion of cell division. It forms part of a complex with these proteins promoting stability and functionality of the cleavage furrow. Anillin's structural role is critical for the maintenance and integrity of the dividing cell's structure.
Pathways
Anillin plays a role in the RHO signaling pathway and the cytoskeleton remodeling pathway. It interacts with other proteins in these pathways such as RhoA and Ect2 facilitating the precise control of contractile ring formation. These interactions recruit additional factors to the cleavage site organizing cellular machinery for the final stages of cell division. Anillin's involvement in these pathways underlines its significance in both cell signaling and structural organization.
Anillin has been correlated with cancer progression and metastasis. Overexpression of Anillin has been observed in several types of cancers including breast and lung cancer. It interacts with proteins such as Aurora B kinase linking Anillin to tumor cell proliferation and survival. Understanding Anillin's function and interactions can offer insights into potential therapeutic strategies for targeting dysregulated cell division in cancer.