Agilent, 5190-2502, Agilent Bio SEC-3, 100Å, 7.8 x 150 mm, 3 µm

Agilent Bio SEC-3, 100Å, 7.8 x 150 mm, 3 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.1-100 kDa.

Specifications:
Brand: Agilent Bio SEC-3
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 150 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 100 Da
Molecular Weight Upper Limit: 100000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 3 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 100 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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The Agilent Bio SEC-3 column, featuring 100 angstrom pore size, 7.8 x 150 mm dimensions, and 3 micron particle size, is expertly designed for high-resolution size exclusion chromatography of biological molecules such as proteins, peptides, and biotherapeutics. Engineered with proprietary particle technology, this column delivers precise molecular weight separation and excellent reproducibility, making it an ideal choice for applications involving protein aggregation, fragment analysis, and purity assessment.

Optimized for aqueous mobile phases, this column is compatible with most HPLC and UHPLC systems, including Agilent 1100, 1200, 1260 Infinity, and 1290 Infinity platforms. It supports a range of flow rates and injection volumes, providing robust performance in both analytical and characterization workflows for research and quality control laboratories.

The column’s inert surface minimizes non-specific interactions, enhancing protein recovery and peak symmetry, and supports analysis of sensitive biomolecules. It is an essential tool for laboratories engaged in biopharmaceutical research, structural biology, and quality assurance of protein therapeutics. Additionally, it can be used alongside other Agilent Bio SEC columns to tailor chromatographic separations across a broader molecular weight range, ensuring consistent, high-quality results in demanding biomolecular analyses.