Agilent, 5190-2521, Agilent Bio SEC-5, 150Å, 7.8 x 300 mm, 5 µm

Agilent Bio SEC-5, 150Å, 7.8 x 300 mm, 5 µm. A size exclusion column for aggregate analysis of peptides and small proteins. Resolving range 0.5-150 kDa.

Specifications:
Brand: Agilent Bio SEC-5
Hardware: SS
Inner Diameter (ID): 7.8 mm
LC Platform: Stainless Steel
Length: 300 mm
Maximum Temperature: 80 °C
Molecular Weight Lower Limit: 500 Da
Molecular Weight Upper Limit: 150000 Da
Part Number Application Domain: Biopharma
Part Number Application: Aggregate & Fragment AnalysisPeptidesProteins
Particle Size: 5 µm
Particle Type: Fully Porous
Phase: SEC
Pore Size: 150 Å
Pressure Rating: 240 bar
Separation Mode: Size Exclusion (SEC)
Shipping Solvent: Phosphate Buffer
Technique: LC
UNSPSC Code: 41115711
pH Range: 2-8.5

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Designed for high-resolution size exclusion chromatography (SEC) of biomolecules, this column features a 150 angstrom pore size and a 7.8 x 300 mm dimension with a 5 micron particle size. It delivers precise separation and accurate molecular weight determination of proteins, peptides, and other biological macromolecules. The robust silica-based material provides excellent chemical stability, reproducibility, and longevity, making it ideal for routine analysis in research and pharmaceutical laboratories.

Common usage scenarios include aggregate and fragment analysis of monoclonal antibodies, characterization of protein therapeutics, and purity assessments for biopharmaceutical development. The column ensures minimal sample adsorption and supports reproducible quantification even at low concentrations. It can be seamlessly integrated with Agilent HPLC and UHPLC systems, such as the Agilent 1260 Infinity II or 1290 Infinity II series, as well as other liquid chromatography instruments compatible with standard column dimensions.

Researchers benefit from fast run times, sharp peak resolution, and reliable performance across diverse sample matrices. The column is also compatible with a range of Agilent SEC standards and mobile phases, enabling comprehensive workflows for protein and aggregate analysis. This product is an essential tool for advancing biotherapeutic research and quality control processes.